[Full Text], Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.rec12146 Dilute stock solution 10:1 to make a 1x working solution. Acetate Buffer Particle Bombardment TBST Buffer ... E. F., and Shambrook, J. [Full Text], Cold Spring Harb Protoc; 2013; doi:10.1101/pdb.rec074104 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec8494 Transfer buffer (wet) 25 mM Tris base; 190 mM glycine; 20% methanol; Check the pH and adjust to 8.3; For proteins >80 kDa, we recommend including SDS at a final concentration of 0.1%. [Full Text], Tyrode Solution for Probing Ca2+ Signaling, Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.rec077859 Discard the pellet. [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec57 Preparation for the 10X TBE Electrophoresis Buffer . Doc western blotting buffer recipes vera ji academia edu how do you dissolve bsa powder recipe of western blot blocking solution table tbst tris buffered saline with tween 20 at … [Full Text]. Store at 4°C. [Full Text], Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.rec12042 [Full Text], Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.rec11098 [Full Text], Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.rec084509 [Full Text], Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.rec087015 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec8323 [Full Text], Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.rec085696 After blocking the membrane for 1 h at room temperature in 5% skim milk in TBST buffer (20 mM Tris–HCl [pH 7.5], 150 mM NaCl, and 0.1% Tween 20), the blot was probed with mouse monoclonal anti-HA primary antibody (Cell Signaling Technology) at a dilution of 1:1000 in the TBST buffer for 4 h at room temperature. [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec629 [Full Text], Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.rec085415 [Full Text], Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.rec11405 [Full Text], Cold Spring Harb Protoc; 2013; doi:10.1101/pdb.rec076687 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec439 Nonfat Dry Milk: . [Full Text], Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.rec11106 [Full Text], Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.rec11948 [Full Text], Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.rec11162 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec8955 [Full Text], Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.rec11299 [Full Text], Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.rec12074 [Full Text], Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.rec100917 [Full Text], Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.rec11647 [Full Text], Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.rec12443 [Full Text], Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.rec10899 Scrape adherent cells off the dish using a cold plastic cell scraper, then gently [Full Text], Tris-buffered saline with detergent (TBSTW), Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.rec11485 Dilute the primary antibody in 15 ml of 5% non-fat dry milk in TBST. [Full Text], Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.rec079574 Google Scholar. [Full Text], Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.rec085134

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